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We report a molecular survey aimed to deepen the knowledge on the genetic variability of an allochthonous population of Southern pike (Esox cisalpinus) present in Sardinia. The analysis of 51 individuals showed high levels of genetic variability: 1) eleven haplotypes were found, 8 of which private to Sardinia (41% individuals); 2) five different mitochondrial lineages were reported, consistent with the release of a conspicuous number of individuals, likely through multiple introduction events; 3) several new haplotypes esclusive to a single individual, diverging from the peninsular haplotypes for one/two point mutations, were evidenced. As 35% individuals from Sardinia share haplotypes exclusively found in Northern Italy, this geographic area has likely been the main source contributing to the gene pool of this alien population.
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